Daniel Zamudio died Tuesday night, 25 days after he was attacked. The case has prompted a national debate in Chile over hate crimes, with President Sebastian Pinera saying from Asia that his government won't rest until a proposed anti-discrimination law is passed.
Four suspects have been jailed on attempted murder charges, some of whom already have criminal records for attacks on gays.
Hours after Zamudio's death, prosecutor Ernesto Vazquez formally requested that the charges be changed to premeditated murder, carrying maximum life sentences if convicted. He said the attack was clearly motivated by homophobia.